Using the Tiff class to read image sequences instead of stacks
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Hi there,
I want to read (as fast as possible of course) multi-dimensionnal (5D) microscopy images (eg. Height x Width x Channels x # of Z planes x # T frames) and making RGB composites from them. Right now I'm using imread inside for loops to read all the images. hFiles is the structure in which I read all the names and store corresponding images to MatrixWithImages.
InfoImages = imfinfo('...name of the first image');
i = 0;
m = 0;
for x = 1:NumberChannels
for y = 1:NumberSlices
for z = 1:NumberFrames
MatrixWithImages(:,:,x,y,z) = imread(hFiles(z+i+m).name,'Info',InfoImages);
end
i = i + NumberFrames;
end
i = 0;
m = m + NumberSlices;
end
% ... code to make composite images .
I would like to take advantage of the Tiff class since it would surely speed up the process for large datasets, however I don't know how to use it to read images stored as sequences instead of stacks. I guess it does not make sense to create one Tiff object for every image in the sequence, so I'm a bit lost.
Can anybody help me on this?
Thanks.
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