How can I identify features in a microscope image

Hi everyone.

I'm trying to measure the sizes of the particles (the small lumps that make up the bigger cluster) in a microscope image. I tried to estimate the sizes of the particles using imfindcircles but it was not very accurate. This is what the image looks like after pre-processing:

I'm new to image processing and MATLAB and would like some help in finding the best way to separate each particle and measure its area. Thanks in advance.

Answer 1

[Image Analyst]

It's not obvious enough to be done automatically therefore I recommend you use imdistline() and manually do it.

[Zul]

I have to do this for a number of images so doing it manually is not an option. But I guess there is no better solution, thanks anyway.