I'm getting this error: Undefined function 'B1GLAM2x2' for input arguments of type 'uint8'. Error in BGLAM_train_ann (line 159) [GLAM1] = B1GLAM2x2(I); %%% Basic Gray Level Aura Matrix (BGLAM)

 FinalIm = uint8(FinalIm);

    figure(9), imshow(FinalIm);title('Segmented image ROI1 (300x300)');

    [m2 n2 d2] = size(FinalIm);
    C1 = imcrop(FinalIm,[125 125 50 50]);
    figure(10), imshow(C1);title('Segmented image ROI2 (50x50)');
    C2 = imcrop(FinalIm,[100 100 100 100]);

    figure(11), imshow(C2);title('Segmented image ROI3 (100x100)');

    % [m3 n3 d3] = size(C);
    I=FinalIm; %%%%(ROI1)
    I1=C1;%%%%(ROI2)
    I2=C2;%%%%(ROI3)

    %%%%%%%image noise removes %%%%%%%%
    [r c loon]=size(I);
    [r1 c1 loon1]=size(I1);
    [r2 c2 loon2]=size(I2);

    width=r; height=c; d=loon;
    width1=r1; height1=c1; d1=loon1;
    width2=r2; height2=c2; d2=loon2;

    for i=1:width
        for j=1:height

            if I(i,j,1)>= 150 && I(i,j,2)>= 150 && I(i,j,3)>= 0

                I(i,j,1)= 0;
                I(i,j,2)= 0;
                I(i,j,3)= 0;

            end;
        end;
    end ;

    for i1=1:width1
        for j1=1:height1

            if I1(i1,j1,1)>= 150 && I1(i1,j1,2)>= 150 && I1(i1,j1,3)>= 0

                I1(i1,j1,1)= 0;
                I1(i1,j1,2)= 0;
                I1(i1,j1,3)= 0;

            end;
        end;
    end ;

    for i2=1:width2
        for j2=1:height2

            if I2(i2,j2,1)>= 150 && I2(i2,j2,2)>= 150 && I2(i2,j2,3)>= 0

                I2(i2,j2,1)= 0;
                I2(i2,j2,2)= 0;
                I2(i2,j2,3)= 0;

            end;
        end;
    end ;
    %
    % figure(12), imshow(I);title('Segmented image ROI3 (100x100)');
    % figure(13), imshow(I1);title('Segmented image ROI3 (100x100)');
    % figure(14), imshow(I2);title('Segmented image ROI3 (100x100)');

    %   end
    % return
    %%%%%%Step5: image featur extraction %%%%%%%%%%%%%%%%

    [GLAM1] = B1GLAM2x2(I); %%%Basic Gray Level Aura Matrix (BGLAM)
    [GLAM2] = B2GLAM2x2(I1);
    [GLAM3] = B3GLAM2x2(I2);
    s1 = size(GLAM1);
    s2 = size(GLAM2);
    s3 = size(GLAM3);

    nbre

    %%%%%%Step6: Normalization %%%%%%%%

    nor=max(GLAM1);
    sample=(GLAM1/nor)';
    nor1=max(GLAM2);
    sample1=(GLAM2/nor1)';
    nor2=max(GLAM3);
    sample2=(GLAM3/nor2)';

[Geoff Hayes]

Muhammad - do you have the function B1GLAM2x2? In the Command Window, type

 which B1GLAM2x2 -all

and see if the path to the function is returned. If not, check to see where this file (presumably it is either a file/function that you created or downloaded from somewhere) is located and add the folder (of this file) to your MATLAB search path.