How can I create transition and emission matrix for all the observation sets in HMM ?

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I have 13 sequences and their corresponding class. I found that for HMM, hmmestimate() takes a seq of 1 row vector and its corresponding states of 1 row vector. And return the transition and emission matrix. But I want to built the transition and emission matrix with the knowledge of all this 13 sequences. How can I do this?

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