how to program a pushbutton and let it import all the tif file

this is how it should be done, but there is some error anyone know what is wrong with the coding?

% --- Executes on button press in Run_btn. function Run_btn_Callback(hObject, eventdata, handles) % hObject handle to Run_btn (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) cd(handles.current_folder); files=dir('*.TIF'); fid = fopen(strcat(handles.current_folder,'\eggResult.txt'),'wt+'); fprintf(fid, '\tFiles_Name\t\t Regression(r) Slope(m)\tOffset(b)\t Result\n\n'); fclose(fid);

maxconbar=waitbar(0,'1','Name','Processing Imgaes',... 'CreateCancelBtn',... 'setappdata(gcbf,''canceling'',1)');

a = 1; for i=1: length(files)

     if getappdata(maxconbar,'canceling')
                break
     end

     b = length(files);
     %Report file being process in waitbar message field
     waitbar(i/length(files),maxconbar,sprintf(strcat( num2str(a) ,'/', num2str(b) ,'   Processing:',char(files(i).name))));
     a = a + 1;
%    disp(strcat('Processing ',char(files(i).name)));

    im0=imread(files(i).name);
    im0=imadjust(im0,stretchlim(im0),[]);
    s=size(im0);
    im0=imcrop(im0,[floor(s(1)/4) floor(s(2)/4) floor(s(1)/2) floor(s(2)/2)]);
%figure,imshow(im0);
    im1=im2bw(im0,graythresh(im0));
%figure,imshow(im1);
    im1=imcomplement(im1);
%figure,imshow(im1);
    im1=imfill(im1,'holes');
%figure,imshow(im1);
    s=size(im1);
    onepercent=floor(s(1)*s(2)*0.01);
    im1=bwareaopen(im1,onepercent);
%figure,imshow(im1);
    imcc=bwconncomp(im1);
    %----------------------------------------------
    Zebrafishdata = regionprops(imcc, 'area');
    Zebrafish_areas = [Zebrafishdata.Area];
    [max_area, idx] = max(Zebrafish_areas);
    percentage=floor(s(1)*s(2)*0.16);
    if max_area>=percentage
        continue;
    else
        Zebrafish = false(size(im1));
        Zebrafish(imcc.PixelIdxList{idx}) = true;
        area{i}= max_area;

        greyvector=im0(imcc.PixelIdxList{1});
        greyvector=sort(greyvector);
%figure,plot(greyvector,'color','r')

        y=double(greyvector);
        y=y';
        L=length(y);
        t=1:L;
        [r,m,b]=regression(t,y);
       %[files_name]=files(i).name;

        fid  = fopen(strcat(handles.current_folder,'\eggResult.txt'),'at+');
        string1='alive';
        string2='dead';
        if m>=0.2250
            fprintf(fid, '%s     %7.5f            %6.4f       %e          %s\n\n',files(i).name,r,m,b,string1);
        else
            fprintf(fid, '%s     %7.5f            %6.4f       %e          %s\n\n',files(i).name,r,m,b,string2);
        end

            fclose(fid);

       if m<0.2250
            save{i}= m;
            D=cell2mat(save);
%             q=plot(handles.axes2,D);           
%             set(q,'Color','red')
%             hold all;

        else
            store{i}= m;
            A=cell2mat(store);
%             p=plot(handles.axes2,A);
%             set(p,'Color','blue')
%             title('Alive(Blue) vs Dead(Red)');

        end       
%figure,plotregression(t,y)

    end
end
delete(maxconbar); 
q=plot(handles.axes2,D);           
set(q,'Color','red')
hold all;

p=plot(handles.axes2,A); set(p,'Color','blue')

set(handles.axes2,'XGrid','on'); set(handles.axes2,'YGrid','on'); title('Alive(Blue) vs Dead(Red)');

message = sprintf('%d images processed.', a-1); uiwait(msgbox(message));

guidata(hObject, handles);

[Jan]

Please format your code properly, when you want others to read it. And explain which error you see. It is very frustrating when you mention, that there is "some error" but do not offer any of the important details. You have the required information already, but all readers have to find out by their own, if you get an error message of if the results differ from your expectations.

Answer 1

[haha haha]