I must recognize two successive frames if a cell is divided or not and know which cell is

We have a set of frames sampled at successive instants of time and we should first be able to track the movement of cells and recognize and report those cells that fulfill mitosis following the movement of the two new cells created from the original.

thanks in advance for your help and advice Luca

[Salaheddin Hosseinzadeh]

Hi Luca,

Sounds like very interesting image processing project.

I like to know the answer, so I left a comment to keep the track of this question.

I am thinking of counting the number of cells in the frames, so if its increased then there has been a division!

[Luca Apolito]

but this way you can not know which is the cell that has been split and in what position is located in the frame!

[Thomas Koelen]

If you don't upload frames there is no way of knowing how to approach this problem.

[Salaheddin Hosseinzadeh]

Ok! How about measuring the perimeter of the cells, if one increased beyond some threshold then it means it is about to divide!

[Luca Apolito]

Actually I need a MATLAB code that allows me to track the movement of cells from 92 frame format ' .tif ' . Not only the movement but also try to figure out which cell divides and save everything to a .csv file . Does anyone have any ideas? ( I do not have too much familiarity with MATLAB so if anyone has some time ... ) thanks a lot

[Salaheddin Hosseinzadeh]

Luca,

Please put some pictures, this will help experts to help you with your problem. I guess there should be something specific about the cell that you can track it among many similar cell!

Put some images, even of the motion, lets see what's going to be the answer. Hopefully someone will notice your question then. I am also interested to know what's the solution.

[Luca Apolito]

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[Luca Apolito]

[Luca Apolito]

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[Luca Apolito]

[Luca Apolito]

TASK:

Segmenting the cells in each frame

Identify the centers and the radii of cells

Implement a code tracking cell

Identify frame with mitosis and cell indices involved

Answer 1

[Image Analyst]

Check out the tracking capabilities in the Computer Vision System Toolbox.

[Luca Apolito]

we would like to implement a code which set the parameters and decision-making with which to save the results of diseased cells with their respective frames that divide can then use with this toolbox. The toolbox implements a number of features that we can not mix

[Image Analyst]

I don't know why you said the last sentence, but anyway, good luck.