<http://in.mathworks.com/matlabcentral/answers/152281-calculate-the-distance-between-two-objects-automatically-using-matlab This> MATLAB Answers post can give some inputs to proceed further.

Distance between two points of an image?

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Deepak M V on 23 Jun 2015

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Answered: Image Analyst on 23 Jun 2015

... please help me

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Walter Roberson on 23 Jun 2015

Did the question get edited away?

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Ganesh Gaonkar on 23 Jun 2015

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This MATLAB Answers post can give some inputs to proceed further.

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Image Analyst on 23 Jun 2015

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spatial_calibration_demo.m

Try my spatial calibration demo.

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