How to calculate the actual area (in Cm^2) for an irregular shape in an image?? Please help me.
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You need to know the size of your image's total field of view, or an object of known size in the image, so that you can calculate a pixels/cm calibration factor. Then calculate area in pixels as usual (bwlabel, regionprops) and multiply by the square of the calibration factor.
There's an app for that: http://hight3ch.com/the-app-that-can-measures-everything-from-your-photos/#more-7597