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a=0.85; for i=1:7 filename=strcat('D:\2nd year\EXP season 2\Dry\cat1\',num2str(i),'.bmp'); I=imread(filename); I1=I(:,:,2); I2=I(:,:,3); G_Channel_cat1(i-2)=mean(mean(I1(293+(-5:5),285+(-5:5))))-mean(mean(I2(293+(-5:5),285+(-5:5))))*a; end
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When i is 1, i-2 is going to be -1, and you cannot store into element number -1 of G_Channel_cat1.
It is not obvious what you are trying to do in that assignment statement, so I cannot suggest any fix other than not subtracting 2 from "i" at that point.