I use Genetic Algorithm for optimization of 6 parameters and they limited between 0-100 with Lower and Upper bounds in constrains but in running time it's also setting a negetive number(out of that range)!Why it's occure?
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It is possible that you aren't setting bounds on all components. See the documentation of bounds.
For example, your lower bounds should be zeros(6,1), and your upper bounds should be 100*ones(6,1).
MATLAB mathematical toolbox documentation