please help me solve this error on GUI

Dot indexing is not supported for variables of this type.

Error in DetectDisease_GUI>pushbutton1_Callback (line 44)

axes(handles.axes1);

Error in gui_mainfcn (line 95)

feval(varargin{:});

Error in DetectDisease_GUI (line 18)

gui_mainfcn(gui_State, varargin{:});

Error in

matlab.graphics.internal.figfile.FigFile/read>@(hObject,eventdata)DetectDisease_GUI('pushbutton1_Callback',hObject,eventdata,guidata(hObject))

Error while evaluating UIControl Callback.

function varargout = DetectDisease_GUI(varargin)

gui_Singleton = 1;
gui_State = struct('gui_Name',       mfilename, ...
                   'gui_Singleton',  gui_Singleton, ...
                   'gui_OpeningFcn', @DetectDisease_GUI_OpeningFcn, ...
                   'gui_OutputFcn',  @DetectDisease_GUI_OutputFcn, ...
                   'gui_LayoutFcn',  [] , ...
                   'gui_Callback',   []);
if nargin && ischar(varargin{1})
    gui_State.gui_Callback = str2func(varargin{1});
end

if nargout
    [varargout{1:nargout}] = gui_mainfcn(gui_State, varargin{:});
else
    gui_mainfcn(gui_State, varargin{:});
end

function DetectDisease_GUI_OpeningFcn(hObject, ~, handles, varargin)

handles.output = hObject;
ss = ones(300,400);
axes(handles.axes1);
imshow(ss);
axes(handles.axes2);
imshow(ss);
axes(handles.axes3);
imshow(ss);

guidata(hObject, handles);


function varargout = DetectDisease_GUI_OutputFcn(~, ~, ~) 

function pushbutton1_Callback(hObject, ~, handles)

clc
[filename, pathname] = uigetfile({'*.*';'*.bmp';'*.jpg';'*.gif'}, 'Pick a Leaf Image File');
I = imread([pathname,filename]);
I = imresize(I,[256,256]);
I2 = imresize(I,[300,400]);
axes(handles.axes1);
imshow(I2);title('Query Image');
ss = ones(300,400);
axes(handles.axes2);
imshow(ss);
axes(handles.axes3);
imshow(ss);
handles.ImgData1 = I;
guidata(hObject,handles);


function pushbutton3_Callback(hObject, ~, handles)

I3 = handles.ImgData1;
I4 = imadjust(I3,stretchlim(I3));
I5 = imresize(I4,[300,400]);
axes(handles.axes2);
imshow(I5);title(' Contrast Enhanced ');
handles.ImgData2 = I4;
guidata(hObject,handles);

function pushbutton4_Callback(hObject, ~, handles)

I6 = handles.ImgData2;
I = I6;
%% Extract Features

cform = makecform('srgb2lab');

lab_he = applycform(I,cform);


ab = double(lab_he(:,:,2:3));
nrows = size(ab,1);
ncols = size(ab,2);
ab = reshape(ab,nrows*ncols,2);
nColors = 3;
[cluster_idx , ~] = kmeans(ab,nColors,'distance','sqEuclidean', ...
                                      'Replicates',3);

pixel_labels = reshape(cluster_idx,nrows,ncols);

segmented_images = cell(1,3);

rgb_label = repmat(pixel_labels,[1,1,3]);

for k = 1:nColors
    colors = I;
    colors(rgb_label ~= k) = 0;
    segmented_images{k} = colors;
end



figure,subplot(2,3,2);imshow(I);title('Original Image'); subplot(2,3,4);imshow(segmented_images{1});title('Cluster 1'); subplot(2,3,5);imshow(segmented_images{2});title('Cluster 2');
subplot(2,3,6);imshow(segmented_images{3});title('Cluster 3');
set(gcf, 'Position', get(0,'Screensize'));
set(gcf, 'name','Segmented by K Means', 'numbertitle','off')

pause(2)
x = inputdlg('Enter the cluster no. containing the ROI only:');
i = str2double(x);

seg_img = segmented_images{i};

if ndims(seg_img) == 3
   img = rgb2gray(seg_img);
end

black = im2bw(seg_img,graythresh(seg_img));

m = size(seg_img,1);
n = size(seg_img,2);

zero_image = zeros(m,n); 


cc = bwconncomp(seg_img,6);
diseasedata = regionprops(cc,'basic');
A1 = diseasedata.Area;
sprintf('Area of the disease affected region is : %g%',A1);

I_black = im2bw(I,graythresh(I));
kk = bwconncomp(I,6);
leafdata = regionprops(kk,'basic');
A2 = leafdata.Area;
sprintf(' Total leaf area is : %g%',A2);

%Affected_Area = 1-(A1/A2);
Affected_Area = (A1/A2);
if Affected_Area < 0.1
    Affected_Area = Affected_Area+0.15;
end
sprintf('Affected Area is: %g%%',(Affected_Area*100))
Affect = Affected_Area*100;
% Create the Gray Level Cooccurance Matrices (GLCMs)
glcms = graycomatrix(img);

% Derive Statistics from GLCM
stats = graycoprops(glcms,'Contrast Correlation Energy Homogeneity');
Contrast = stats.Contrast;
Correlation = stats.Correlation;
Energy = stats.Energy;
Homogeneity = stats.Homogeneity;
Mean = mean2(seg_img);
Standard_Deviation = std2(seg_img);
Entropy = entropy(seg_img);
RMS = mean2(rms(seg_img));
%Skewness = skewness(img)
Variance = mean2(var(double(seg_img)));
a = sum(double(seg_img(:)));
Smoothness = 1-(1/(1+a));
Kurtosis = kurtosis(double(seg_img(:)));
Skewness = skewness(double(seg_img(:)));
% Inverse Difference Movement
m = size(seg_img,1);
n = size(seg_img,2);
in_diff = 0;
for i = 1:m
    for j = 1:n
        temp = seg_img(i,j)./(1+(i-j).^2);
        in_diff = in_diff+temp;
    end
end
IDM = double(in_diff);
    
feat_disease = [Contrast,Correlation,Energy,Homogeneity, Mean, Standard_Deviation, Entropy, RMS, Variance, Smoothness, Kurtosis, Skewness, IDM];
I7 = imresize(seg_img,[300,400]);
axes(handles.axes3);
imshow(I7);title('Segmented ROI');
%set(handles.edit3,'string',Affect);
set(handles.edit5,'string',Mean);
set(handles.edit6,'string',Standard_Deviation);
set(handles.edit7,'string',Entropy);
set(handles.edit8,'string',RMS);
set(handles.edit9,'string',Variance);
set(handles.edit10,'string',Smoothness);
set(handles.edit11,'string',Kurtosis);
set(handles.edit12,'string',Skewness);
set(handles.edit13,'string',IDM);
set(handles.edit14,'string',Contrast);
set(handles.edit15,'string',Correlation);
set(handles.edit16,'string',Energy);
set(handles.edit17,'string',Homogeneity);
handles.ImgData3 = feat_disease;
handles.ImgData4 = Affect;
% Update GUI
guidata(hObject,handles);

function edit2_Callback(~, ~, ~)

function edit2_CreateFcn(hObject, ~, ~)

if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit3_Callback(~, ~, ~)

function edit3_CreateFcn(hObject, ~, ~)

if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end

function edit4_Callback(~, ~, ~)

function edit4_CreateFcn(hObject, ~, ~)

if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end


% --- Executes on button press in pushbutton6.
function pushbutton6_Callback(hObject, ~, handles)

test = handles.ImgData3;
Affect = handles.ImgData4;
% Load All The Features
load('Training_Data.mat')

% Put the test features into variable 'test'

result = multisvm(Train_Feat,Train_Label,test);
%disp(result);

% Visualize Results
if result == 0
    R1 = 'Alternaria Alternata';
    set(handles.edit2,'string',R1);
    set(handles.edit3,'string',Affect);
    helpdlg(' Alternaria Alternata ');
    disp(' Alternaria Alternata ');
elseif result == 4
    R5 = 'Healthy Leaf';
    R6 = 'None';
    set(handles.edit2,'string',R5);
    set(handles.edit3,'string',R6);
    helpdlg(' Healthy Leaf ');
    disp('Healthy Leaf ');
end
% Update GUI
guidata(hObject,handles);

% --- Executes on button press in pushbutton7.
function pushbutton7_Callback(~, ~, ~)

close all


function edit5_Callback(~, ~, ~)

function edit5_CreateFcn(hObject, ~, ~)

if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit6_Callback(~, ~, ~)

function edit6_CreateFcn(hObject, ~, ~)

if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit7_Callback(~, ~, ~)
% hObject    handle to edit7 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Hints: get(hObject,'String') returns contents of edit7 as text
%        str2double(get(hObject,'String')) returns contents of edit7 as a double


% --- Executes during object creation, after setting all properties.
function edit7_CreateFcn(hObject, ~, ~)
% hObject    handle to edit7 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called

% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit8_Callback(~, ~, ~)
% hObject    handle to edit8 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Hints: get(hObject,'String') returns contents of edit8 as text
%        str2double(get(hObject,'String')) returns contents of edit8 as a double


% --- Executes during object creation, after setting all properties.
function edit8_CreateFcn(hObject, ~, ~)
% hObject    handle to edit8 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called

% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit9_Callback(~, ~, ~)
% hObject    handle to edit9 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Hints: get(hObject,'String') returns contents of edit9 as text
%        str2double(get(hObject,'String')) returns contents of edit9 as a double


% --- Executes during object creation, after setting all properties.
function edit9_CreateFcn(hObject, ~, ~)
% hObject    handle to edit9 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called

% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit10_Callback(~, ~, ~)
% hObject    handle to edit10 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Hints: get(hObject,'String') returns contents of edit10 as text
%        str2double(get(hObject,'String')) returns contents of edit10 as a double


% --- Executes during object creation, after setting all properties.
function edit10_CreateFcn(hObject, ~, ~)
% hObject    handle to edit10 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called

% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit11_Callback(~, ~, ~)

function edit11_CreateFcn(hObject, ~, ~)

if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit12_Callback(~, ~, ~)

function edit12_CreateFcn(hObject, ~, ~)


if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit13_Callback(~, ~, ~)

function edit13_CreateFcn(hObject, ~, ~)

if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit14_Callback(~, ~, ~)

function edit14_CreateFcn(hObject, ~, ~)

if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit15_Callback(~, ~, ~)

function edit15_CreateFcn(hObject, ~, ~)

if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end



function edit16_Callback(~, ~, ~)

function edit16_CreateFcn(hObject, ~, ~)

if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end

function edit17_Callback(~, ~, ~)

function edit17_CreateFcn(hObject, ~, ~)

if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end

[Mehmed Saad]

insert breakpoint at line 44 and check what is inside the variable handles. Dot index is not supported for variable handles which means it doesnot contain content you are trying to access or its type is different.

[Geoff Hayes]

krishna - since this is a GUIDE GUI, how have you launched it? If you have double-clicked or opened the DetectDisease.fig file then you may see an error like this. GUIDE GUIs can only be launched using the DetectDisease.m file by running it from the command line, or in the MATLAB file editor, or from the GUIDE editor. You cannot launch the GUI using the fig file.