Empty detection results with Faster R-CNN - help!!

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I am trying to train a Faster R-CNN 2D but I always get empty detection results...
  • I use a pre-trained backbone network on my data (which has an accuracy of around 70%);
  • The images have size [512 512 1] and are uint8 (as well as the input size of the network);
  • The bounding boxes are approximately between 30x30 to 60x60;
  • I have 2 classes of objects;
  • 250 epochs (already varied it but the result is the same) with MB size 64;
  • I've tried it with a very low positive overlap range ([0.1 1]);
  • ROI Mas pooling 7x7
  • I used fasterRCNNLayers to create a faster R-CNN object.
(500 images+bounding boxes around objects of two classes to train)
Example of training data table:
imageFilename class1 class2
'image1.tiff' [100, 110, 35, 50] []
'image2.tiff' [] [50,20, 40,30]
1) Is there a problem with the images being grayscale and not in color?
2) Do I have to have bounding boxes from a region other than the object? (as described here: https://www.mathworks.com/matlabcentral/answers/500950-bounding-box-not-drawn-some-variables-are-empty).
I really need some help... I don't understand how it runs without errors and then it can't find any object.
Thank you so so much in advance!!!

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