virtualPCR v0.6
by Gie Spaepen
14 Jan 2007
(Updated 17 Oct 2007)
This little program generates real-time PCR plots based all important real-time PCR parameters.
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Watch this File
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| File Information |
| Description |
Install: unzip and put all the files in one directory.
Use: Fill in the amount of cDNA (ng), dNTPs (ng), primers/probe (ng), the efficiency of the primer-probe, the quality of the RNA (RIN-number), the name of the sample and the number of cycles. Press 'Add Sample' to add it to the list. After adding all the samples press 'Calculate' to start the reaction. An image of the amplification plot can be saved in the directory of the program and the results can be seen in the command window by clicking 'Visualize results'. If you want to remove samples click 'Clear samples'. The scale of the Y-axis can be changed by using the radio buttons on the left. The listbox shows the samples list which were added.
The program is based on the basic kinetics of a pcr reaction. See doRTReaction.m for the algorithm and the Readme.pdf file for more info. |
| MATLAB release |
MATLAB 7.0.1 (R14SP1)
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| Updates |
| 23 Jan 2007 |
For an easier use, some features were added to the GUI for sample handling and axis handling. Some minor bugs were also removed. |
| 17 Jul 2007 |
Adaption of the algorithm. The concentration of primers and probes is now, in contrast to previous version, taken into account. |
| 16 Oct 2007 |
The initial degradation of the source RNA influences the real-time PCR result. This effect is now included. |
| 16 Oct 2007 |
Updated the screenshot. |
| 17 Oct 2007 |
Updated ZIP-Package |
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Contact us at files@mathworks.com
