Code covered by the BSD License  

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FRETSCAL

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FRETSCAL

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For the FRET analysis of images of yeast cells labeled with YFP and CFP.

frestoredefaults
function frestoredefaults
%Version 4/3/09 This program restores the original default settings that
%ship with program. Gets called by Fretscal>Summary>Restore Program
%Default settings
global strainname CFPtagname YFPtagname AOIshape CFPspill YFPspill
global frettype cfptype yfptype primary fontpics context toobright
global initialfile culledfile inspectedfile finalfile
global initials culls inspects finals searchlimited culllimited limit1 limit2
global intensitylimit maximumpeaks bringthick bringincr separation maxmovement
global  inputdirectory outputdirectory progbarcol 
global YFPFWHM FRETFWHM CFPFWHM YFPmono FRETmono CFPmono YFPs2b FRETs2b CFPs2b
global YFPFWHMcull FRETFWHMcull CFPFWHMcull YFPmonocull FRETmonocull CFPmonocull YFPs2bcull FRETs2bcull CFPs2bcull
global bitdepth Ysuffix Fsuffix Csuffix Dsuffix bpfilter patchpixel

progbarcol = 'red';
frettype  = 1;   cfptype   = 0;   yfptype   = 0; primary = 1;
strainname = 'unassigned';
CFPtagname = 'CFP_Tagged_Protein';
YFPtagname = 'YFP_Tagged_Protein';
intensitylimit = .50;
inputdirectory = pwd;
outputdirectory = 'out1';
initialfile = 'initial.txt';
culledfile = 'culled.txt';
inspectedfile = 'inspected.txt';
finalfile = 'final.txt';
initials = 0; culls = 0; inspects = 0; finals = 0;
YFPFWHM  = 17;         FRETFWHM = 24;        CFPFWHM  = 24;
YFPmono   = .05;       FRETmono  = 0.5;      CFPmono   = 0.5;
YFPs2b    = 1.3;       FRETs2b   = 1.0;      CFPs2b    = 1;
YFPFWHMcull  = 17;     FRETFWHMcull = 24;    CFPFWHMcull  = 17;
YFPmonocull   = 0.05;  FRETmonocull  = 0.5;  CFPmonocull   = 0.5;
YFPs2bcull    = 4.0;   FRETs2bcull   = 1.0;  CFPs2bcull    = 1;

CFPspill = 0.56;
YFPspill = 0.09;
AOIshape = 19;
bringincr = 5;
bringthick = 3;
separation = 15;
maximumpeaks = 30;
fontpics = 10;
context = 40;
toobright = 0.90;
bitdepth = 16;
Ysuffix = '500_545';
Fsuffix = '440_545';
Csuffix = '440_480';
Dsuffix = '0_500';
bpfilter = 0;
patchpixel = 14000;
maxmovement = 3;
searchlimited = 1;
limit1 = 1;
culllimited = 0;
limit2 = 0;




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