abfload

abfload imports data in the Axon abf format (three different recording modes).

Description

abfload imports data in the Axon abf format. Data may have been acquired in the following modes:

(1) event-driven variable-length
(2) event-driven fixed-length
(3) gap-free

The major output variable d may be a 2D array, a 3D array or a cell array, depending on the recording mode. Information about scaling, the time base (that is, the sampling frequency), and the number of channels and episodes is extracted from the header of the abf file. For details, see help text of abfload.
** PLEASE NOTE: abfload can only deal with abf versions <2 (files acquired with pclamp versions <10). Use abf2load (see below) for all abf versions including the new ones.

• Acknowledgements: This submission has inspired the following:; abf2load
• MATLAB release: MATLAB 6.5.1 (R13SP1)

[Jim Chambers]

works great...I am using it to generate IV curves in real time. Very nice.

[Joanna Huttunen]

Excellent for older types of .abf files. For files collected with Axoscope 10, it does not work... =(

[Harald Hentschke]

Please note: abfload will not work with data acquired with versions 10.x (and up) of pClamp/Axoscope because of major changes in the header structure. There is no way these changes can be accomodated as details of the header structure (which are essential for direct header access into abf files) will no longer be made publicly available.

[Carlos E]

The ABF variable nDataFormat is of type 'short' for version 1.8 of the ABF header. abfload uses an int and so it reads more than it should when trying to open ABF 1.8 files. Usually it just fails. Therefore if you want to open and ABF 1.8 file just change the line 'nDataFormat',100,'int',-1; to use short instead of int then it should work.

[Carlos E]

On a related note, the latest versions of pCLAMP/Axoscope 10.x can export ABF2 files as ABF 1.8 files

[Yoav Mor]

I have found that it is not the nDataFormat that needs to be changed to "Short", rather it is the "nADCNumChannels". Changing this to Short fixes a range of problems with reading abf files, up to version 1.83 (that's what I tested).

[B A]

Great function.
It would be really useful to use 'single' (or even 'int16') variable type for loading in data. This should be more then sufficient for the 12bit encoding in abf files and would save a substantial amount of space. doubles are 64bit and I routinely run out of memory in matlab when using abfload larger files.
Suggestions are welcome.

[D H]

I partially ported this code to python, see http://bitbucket.org/pycurveanal/cagui/wiki/abfload

[Wojciech]

 

[Praneeth Namburi]

Great utility! thank you :)

[Rami]