I compared the ReactionCurve.m output response to various other methods i.e. Sundaresan, K.R. and Krishnaswamy, P.R. (1978) method, the manual point of inflection by inspection method and the two-point method from Smith, C.L. (1972)all of which produced a better fit to my plant model. The best fit was produced from the Sundaresan, K.R. and Krishnaswamy, P.R. (1978) method.