Shortest_route returns an error when called. May be you forgot to submit the Shortest_route.fig file. But, please, before you resubmit, please add a more informative help to your m-file. As it stands now, I only know from the text above, that a adjacency matrix is required.
>> Shortest_route
??? Error using ==> load
Unable to read file Shortest_route.fig: No such file or directory.
Error in ==> hgload at 43
fileVars = load(filename,'-mat');
Error in ==> openfig at 72
[fig, savedvisible] = hgload(filename, struct('Visible','off'));
Error in ==> gui_mainfcn>local_openfig at 286
gui_hFigure = openfig(name, singleton, visible);
Error in ==> gui_mainfcn at 159
gui_hFigure = local_openfig(gui_State.gui_Name, gui_SingletonOpt, gui_Visible);
Error in ==> Shortest_route at 19
gui_mainfcn(gui_State, varargin{:});