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From: ImageAnalyst <imageanalyst@mailinator.com>
Newsgroups: comp.soft-sys.matlab
Subject: Re: find Spots in the Image
Date: Mon, 21 Sep 2009 03:15:00 -0700 (PDT)
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On Sep 21, 12:36 am, "Yi " <heyinba0...@hotmail.com> wrote:
> gray-level
> please help!!

---------------------------------------------------------------------------
Try my image processing tutorial.  It will do this.  All you have to
do is a slight adaptation of it so that the threshold is 0 (or
whatever the brightest gray level of the black spots is).

http://www.mathworks.com/matlabcentral/fileexchange/25157

Good luck,
ImageAnalyst