Thread Subject:

Pixel or Voxel size

I have an image from microscope and I am trying to get quantitative information out of the image.I need to know what is the size of 1 pixel or 1 voxel? So that I can get accurate information out of the image.

"mat001" wrote in message <j5fj6r$6l0$1@newscl01ah.mathworks.com>...
> I have an image from microscope and I am trying to get quantitative information out of the image.I need to know what is the size of 1 pixel or 1 voxel? So that I can get accurate information out of the image.

Easiest way is to be 100% sure is to get a hold of a stage micrometer, and take a picture of it at the same magnification. Then you can count the number of pixels between a known distance.
This information may be stored in your image IF your system has been calibrated correctly, and IF the software you took the image with stores that in the image file. Look around in the acquisition software or the Matlab function IMFINFO if the raw image format can be read directly by Matlab.