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Thread Subject:
Cell detection and segmentation - any advice appreciated!

Subject: Cell detection and segmentation - any advice appreciated!

From: Ess

Date: 18 Apr, 2013 14:12:10

Message: 1 of 1


Recently, I've been using and writing code in Matlab to perform segmentation and tracking of cells in images. Guides such as the the one here ( have been very useful for some image and cell types, but there is one image sequence in particular that I am struggling with. Here is an example of an image from the sequence:

As you can see, there are a few reasons why the segmentation here is tricky; the intensity values of the insides of the cells are very similar to the outside of the cells, and the cell boundaries are often poorly defined.

So far, I've tried using combinations of intensity thresholding followed by various morphological operations, edge detection, thresholding of gradient strength maps, and the recently added activecontour function, to little avail. As I'm also interested in tracking and have multiple frames available, I tried using the outline suggested in, but I have had no luck in getting a reliable output.

Does anyone know of a way to successfully segment an image like this?


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