How to obtain slop for a curve at each point??

I want to estiamte the slop of curve for every point. I attached curve data .

Answer 1

[KSSV]

load bw2.mat ;
x = N(:,1) ;
y = N(:,2) ;
m = gradient(y)./gradient(x) ;

[Surendra Ratnu]

Thanks its works for me, Can you tell me how will i will identify max change in slop and draw the points on curve ??

[KSSV]

Get the differences of slopes using diff. Pick the maximum value out of it.