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What method can I use for background subtraction before performing analysis on my image?

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Hi, i am exploring matlab methods for personal growth and i want to determine how to:
perform background substraction
contrast correction, gaussian filtering and thresholding and binarization for my image.
I have attached a demo m file and image and i get this error:
Unrecognized function or variable 'lowThreshold'.
Error in testfiltering (line 53)
mask = grayImage > lowThreshold & grayImage < highThreshold;

Accepted Answer

Image Analyst
Image Analyst on 15 Jan 2023
You need to assign that value. See the interactive thresholding program in my File Exchange for how to do that.
  3 Comments
Image Analyst
Image Analyst on 16 Jan 2023
I don't know how to answer this. If you started with an image and a background image, you would certainly know if your code did a background subtraction. If you don't have the original image and the background image, then where did you get the image that you have?
"how do I know if the thresholidng i see matches the thresholding i want?" I don't know what this means. It MUST match the threshold you want. If it was not what you wanted, then why did you accept it? Obviously you're only going to accept a threshold that you want. You're not going to accept a threshold that you do not want, of course. That said, it may be that there is no threshold value that will give you a perfect segmentation just by thresholding alone.
Neo
Neo on 16 Jan 2023
i see. yes, this was not a well thought out response from me. i accept your answer and will adjust to my current code,

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