constrain watershed segmentation to existing boundaries

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Hi --
I've defined the basic (and correct) perimeters for some tree canopy objects http://bit.ly/tfl12P. I would like to perform marker-controlled watershed segmentation that is constrained by these boundaries.
Here is my current result: http://bit.ly/tKG1P2. As you can see many objects become much smaller.
Does anyone know of a way to just segment within a defined perimeter?
Thank you for any insight, Mike

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