Previously accessible file is now inaccessible, but using GUIDE

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Hi,
I'm coding an app that ties together a few functions and scripts to decode some data. I've developed the GUI front end using GUIDE, and when I test it before packaging it up using the deploytool, it works fine. However, once the app is packaged up, I get the error,
"Previously accessible file is now inaccessible"
The function file it barfs on is called within another function, which is called from a callback from a button press on the GUI. I've checked, and it's packed up with the rest of the files in the deploy tool. I'm guessing it might be a paths thing, but there aren't any addpath or rmpath calls.
It's driving me mad, as I'm guessing it's something silly during deployment.
Any help would be very much appreciated!

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