Is it possible to set a maximum size of watershed regions? Or easily divide the regions after watershedding?

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I am doing some cell segmentation. I have a code that finds groups of cells with fairly high accuracy. Once the regions are found I use watershedding to separate touching cells. This also works well, however some cells are definitely too large to be just one cell. My question is: Can I set a maximum size each watershed region can be so that larger clumps will split into smaller ones?
Note: I do not care about the number of cells on their own (otherwise I would just divide each region's area by the maximum cell area). I want to separate these clumps so that I can determine how close cells are to the airways (yellow) in the image (i.e. how many cells "belong" to each airway).
I have attached my original image as well as the processed image with the cell regions and airways overlayed in blue and yellow respectively.
Thank you in advanced for the help!
Eric

Answers (1)

Image Analyst
Image Analyst on 12 Jul 2018
Not sure how you define distance. Would bwdist() help? Or how about the Hausdorf distance https://en.wikipedia.org/wiki/Hausdorff_distance ?
Also not sure how you define which yellow blob the blue blobs "belong" to.
  4 Comments
Eric Chadwick
Eric Chadwick on 18 Jul 2018
Edited: Eric Chadwick on 18 Jul 2018
Hi there.
I checked out these links, but I can't seem to get them to work for my problem. I've created the seeds I want to use for watershed, but whenever I try the methods you suggest, I get a blank image. I have attached my image of seeds as well as the original I am trying to watershed.

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