blob-detector

LoG blob detection

blob-detector

basic LoG blob detection.

now plus DoG option

loosely following trackmate

blobdetect

LoG (Laplacian of Gaussian) detector suitable for objects with user-estimated uniform diameter.

Usage

blobcoords=blobdetect(image,diameter)

Arguments

image greyscale image / 2D array.

diameter Diameter of expected objects (in pixels).

Optional keyword arguments

Filter select type of filter, from "LoG", "DoG", default "LoG".

DarkBackground By default bright objects on dark background are expected, if the image instead shows dark objects on light background set this to false. Default true.

MedianFilter The median filter prepocessing step improves quality, default true.

OverlapFilter Eliminate circles overlapping by more than radius. default true.

KernelSize Size of LoG kernel matrix (gets rounded up to nearest odd number). Default 9.

Output

blobcoords [xs, ys] coordinates of blob centers.

annotate_image

Simple utility to inspect output, adds magenta circles to the image.

image_out=annotate_image(image, coords, diameters)

This returns an RGB array. Alternatively, for immediate display as a figure, you could use matlab's viscircles.

Not yet implemented

• handle image edges
• automatically adjust kernel size