thresholding_demo.m interactively loads an image stack, performs thresholding based on the Laplace operator and extracts time-series information from the image stack based on a binary mask provided by the thresholding operation.
mthresh.m performs thresholding based on the Laplace operator.
load3d.m loads an image stack into a 3D array.
zstack.m retrieves z information from a 3D array, based on a binary mask.
These functions provide the core functionality of SARFIA, which was originally developed for Igor Pro.