Research paper by: Daniel Ramot1, Brandon E. Johnson, Tommie L. Berry, Jr, Lucinda Carnell, Miriam B. Goodman
MATLAB Code download available at http://wormsense.stanford.edu. Open source project at http://sourceforge.net/projects/wormtracker
Our tracking system generates a consistent measurement of locomotion that allows direct comparison of results across experiments and experimenters and provides a standard method to share data between laboratories. The tracker utilizes a video camera attched to a zoom lens and a software package implemented in MATLAB®. We demonstrate several proof-of-principle applications for the tracker including measuring speed in the absence and presence of food and in the presence of serotonin. We further use the tracker to automatically quantify the time course of paralysis of worms exposed to aldicarb and levamisole and show that tracker performance compares favorably to data generated using a hand-scored metric.