STORM-based Relative Localization Analysis

Quantify relative localization of proteins based on STORM-based single molecule positional data

Super-resolution STochastic Optical Reconstruction Microscopy (STORM) achieves resolutions of 20 nm laterally and <50 nm in the z-dimension and yields 3D positions of individual molecules. STORM-based Relative Localization Analysis (STORM-RLA) identifies clusters of localized molecules based on 3D particle density and quantifies the spatial relationship between clusters of co-labeled proteins: 1) the instance and degree of overlap between clusters, and, 2) the distance from each cluster to the nearest cluster of the co-labeled protein.