This is not really a MATLAB question, but rather a statistical one. I'll try to point you in the right direction, but you will need to do some learning on your own.
Data, by itself, is not statistically significant. Generally speaking, what may or may not be statistically significant would be a result (e.g. "the mean of these data is larger than zero"), and statistical significance refers to the likelihood that that result might happen due to randomness, rather than a true effect (under certain assumptions).
I recommend you read the Wikipedia page on statistical signficance as a starting point to understand the concept. I strongly advise against trying to implement something in MATLAB when you have such a weak understand of the concept. I'm sorry if that sounds harsh, but it is really the best advice I can give you.
