number of WBC's in an image based on size.

i have written a code to find the number of WBC's in an image based on size. But once the WBC's are highlighted the imfindcircles is not finding it and it is not getting counted.

sum=0;
for l= 104:104
jpgFilename = strcat('2_images_', num2str(l), '.jpg');
wbc=imread('2_images_104.jpg');
figure(1);
imshow(wbc);
title('original image');
%%converted into gray scale
wbc1=rgb2gray(wbc);
 %%thresholding
 T=64; 
[m n]=size(wbc1)
for i=1:m
    for j=1:n
       if wbc1(i,j)>T
           wbc1(i,j)=255;
       else
           wbc1(i,j)=0;
       end
    end
end
figure(2);
imshow(wbc1);
title('thresholded');

%%complement the image
wbc2=imcomplement(wbc1);

%%erosion of the image
A1 = strel('diamond',1);
A=imerode(wbc2,A1);
 A2=imerode(A,A1);
 A3=imerode(A2,A1);
figure(3);
imshow(A3);
title('eroded');

%%using edge detection
BW1 = edge(A3,'canny');
figure(4);
imshow(BW1);
title('edge detection');

A1=strel('diamond',3);
BW2=imdilate(BW1,A1);
BW3=imdilate(BW1,A1);
 BW4=imdilate(BW3,A1);
figure(5);
imshow(BW4);
title('dilated');

BW5=imfill(BW4,'holes');

%%counting the number of WBC
[centers, radii, metric] = imfindcircles(BW5,[1 2],'ObjectPolarity','dark','Sensitivity',0.86,'Method','twostage');
m = viscircles(centers,radii);
[a,b]=size(centers);
fprintf('the number of WBCs in an image %d\n',a);
sum=sum+(a);
end
fprintf('the total number of WBCs in all images %d',sum);

this is the code. please help me

[KALYAN ACHARJYA]

Please attach sample image.

[Guillaume]

And explain what a WBC is, for those of us who have no idea what this stands for.

[Star Strider]

WBC = White Blood Cell most likely, of which there are five principal types, all of them having different staining characteristics, and so little morphological similarity.

[Guillaume]

Thanks, Star.

[Star Strider]

My pleasure.

This is an area of my (occasionally relevant) expertise.

[Bhavna Rajasekaran]

Offcourse its unclear how the wbc image looks like or the final BW5 image that is the input to imfindcircles.. That could rather help in answwering this question better...

Have you tried regionprops for finding centroids of WBC?

[Image Analyst]

Attach an original gray scale image if you still have problems.

How to get a fast answer

Answer 1

[Harshal Faldu]

Thank you for this ,

Please can you give something about how to count types of WBC in the blood image