This toolbox includes routines for using principal component analysis (PCA) and independent component analysis (ICA) to extract cellular signals from imaging data sets. A full description and validation of the method is provided in the paper, "Automated Analysis of Cellular Signals from Large-Scale Calcium Imaging Data," Neuron, 63:747 (2009): http://tinyurl.com/cellsort
Dear Phanindra - You can find sample code that will illustrate the appropriate parameters here:
The processing time depends on the size of your data set. The majority of computation time is in the PCA dimensional reduction pre-processing; subsequent cell sorting is relatively fast.
I need to image segment a tiff calcium image data.I am unable to correctly specify the parameters smwidth and thresh? Can u please tell me what are the typical values? Also how much time does it take to run the whole code till segmentation?
What does movm and movtm mean?
Hi Alex - The CellSortPCA function requires a TIFF image stack.
What file input is necessary for CellSortPCA to work?
The associated publication in Neuron 63, 747–760 makes this look promising. However, getting this to run based on the instructions provided in the supplementary publication is very trying for those with less than a mastery of Matlab.
These functions are well presented and a lot of care has gone into the code. Well worth a look. However, I have had trouble getting this to work reliably with my data since it has tendency to identify noise as the signal.
June 2016: Fixed an inconsistency in CellsortPCA between create_tcov and
Fixed a bug affecting performance in recent versions of MATLAB.
Fixed a minor typo in documentation for CellsortICA - EAM 11/24/09