RatioImage

Create a ratiometric image with intensity modulated display (IMD)

This app will compute and display an intensity modulated ratiometric image. Choose a numerator and denominator image. The pixel-by-pixel ratio image will be displayed via a hue indicated by the colormap, which is user-selectable. The color brightness, however, will be modulated by the total image intensity at that pixel location. Thus, you can make ratiometric images without having to apply thresholding. Adjust the max/min displayed ratio as well as the max/min displayed intensity via sliders below and to the right of the image. Useful for FRET, Calcium ion, or other ratiometric imaging modality.