fca_readfcs

Read FCS 2.0, FCS 3.0 and FCS3.1 format flow cytometry files

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Cite As

Laszlo Balkay (2026). fca_readfcs (https://www.mathworks.com/matlabcentral/fileexchange/9608-fca_readfcs), MATLAB Central File Exchange. Retrieved .

Acknowledgements

Inspired: Ratiometric FRET, CytoMAP

Categories

Find more on Data Import and Analysis in Help Center and MATLAB Answers

General Information

MATLAB Release Compatibility

  • Compatible with any release

Platform Compatibility

  • Windows
  • macOS
  • Linux
Version Published Release Notes Action
2020.6.22

Update to read FCS3.1 data from BD FACS Melody.

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2020.4.11

Update to read FCS3.1 data from Biorad ZE5

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2017.11.1

Minor update to read FCS3.1 data of Attune NxT

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2017.6.1

The code was modified to read FCS 3.1 file.

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1.12.0

The FCS 3.0 standard enables the mixture of word lengths in the data, this upgrade modified the code according to. The linefeed (ASCII code 10) as
the mnemonic separator was also added.
Thanks for William Peria /Fred Hutchinson Cancer Researc

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1.11.0

More accurate compensation correction, gain scaling and appropriate reading for large file size are added. Thanks for Rachel Finck; Garry Nolan's lab at Stanford University and Andrea Pagnani; Politecnico Torino, Human Genetics Foundation.

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1.9.0

The fluorescence compensation has been implemeted into the code. Thanks for Rick Stanton, J. Craig Venter Institute, La Jolla, San Diego

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1.8.0

The fluorescence compensation has been implemented into the code. Thanks for Rick Stanton, J. Craig Venter Institute, La Jolla, San Diego

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1.6.0

Updated to read Luminex 100 data file. Thanks for Ofir Goldberger, Stanford University

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1.5.0

Additional minor changes on the help section.

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1.3.0

The help section of the m file was modified.

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1.2.0

Updated to read the Partec CyFlow format file. Thanks for
Gavin A Price

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1.1.0

Updated to read the Partec CyFlow format file. Thanks for
Gavin A Price

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1.0.0

Updated to read the BD LSR II file format and including the comments of Allan Moser (Cira Discovery Sciences, Inc.) Thanks!

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