How to create ground truth images for comparative analysis

I am working on medical images we have our own medical image data. While I want to compare different de-noising filters or segmentation algorithm I needed some ground truth images. Is there exist such techniques that can generate ground truth images. If so please share link.

[Walter Roberson]

Duplicates http://uk.mathworks.com/matlabcentral/answers/253596-how-to-create-ground-truth-images