Error message when analysing FluoroSNNAP segmentation.

I have a manually created segmentation of live-cell calcium imaging data from neural stem cells. The segmentation worked in FluoroSNNAP and saved sucessfully to the experiment folder. I can open the segmentation in FluoroSNNAP and see the red outlines over my black and white image of my cells, also the rainbow coloured segmentation image. When I ask FluroSNNAP to analyse this segmentation I get many lines of error codes. Starting with "unable to perform assignment because dot indexing is not supported for variables of this type". ThenError in AnalyzeData (line 26)

files.name = files;

Error in FluoroSNNAP>single_file_Callback (line 798)

AnalyzeData({handles.curr_folder},handles.curr_file);

Error in gui_mainfcn (line 95)

feval(varargin{:});

Error in FluoroSNNAP (line 42)

gui_mainfcn(gui_State, varargin{:});

Error in

matlab.graphics.internal.figfile.FigFile/read>@(hObject,eventdata)FluoroSNNAP('single_file_Callback',hObject,eventdata,guidata(hObject))

Error while evaluating Menu Callback.

Error using PostProcess (line 48)

analysis.mat does not exist in search path D:/200611/002TIF/analysis.mat. Please run AnalyzeData.m first

Error in FluoroSNNAP>batchanalysis_Callback (line 784)

PostProcess(handles.folders);

Error in gui_mainfcn (line 95)

feval(varargin{:});

Error in FluoroSNNAP (line 42)

gui_mainfcn(gui_State, varargin{:});

Error in

matlab.graphics.internal.figfile.FigFile/read>@(hObject,eventdata)FluoroSNNAP('batchanalysis_Callback',hObject,eventdata,guidata(hObject))

Error while evaluating Menu Callback.

This segmentation file was created in FluoroSNNAP when used as a plugin for matlab - but matlab refuses to recognise it.

Does anyone know what the problem here is????

[yusmalb]

Hello, did you solve this problem? I also encountered it during the calcium signal processing and would like to consult how you solved it.

[Avni]

Hi,

I have the same problem. Could you please let me know how you solved this indexing issue.

Thank you :)

[Anastasia]

I also have the same problem. Did anyone manage to solve it?