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About brightness

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[EDIT: 20110523 17:15 CDT - reformat. clarify - WDR]
Hello everyone, I have a trouble with the brightness in a pic that I take:
I'm new in Matlab anyone can help me please? I need to remove the brightness in the edges, then to binarize just to see the white points, the picture is of bacterial colonies. Thanks!
PS: sorry for my bad English.

Accepted Answer

Walter Roberson
Walter Roberson on 23 May 2011
I suggest im2bw perhaps in combination with graythresh
  1 Comment
Andre Asfar
Andre Asfar on 23 May 2011
Thanks!, I will try

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