How to compute thickness of a layer in an image

I would like to compute the thickness of a layer in an image (image_1.png). I would like to do this by computing the pixel value in the top layer subtracted by the pixel value in the corresponding bottom layer and averaged over the entire layer . Can someone help me how i could proceed with this?

Thank you

Answer 1

[Simon Chan]

You can only measure the thickness (or distance) in terms of pixel numbers according to your method.

Hence you also need to know the pixel size on your image so that you can convert the pixel value to a real distance.

You may use function imfinfo to retrieve the information regarding to the graphic file

info = imfinfo(filename)

On the other hand, if you are using dicom file, you may use function dicominfo

info = dicominfo(filename)

[Tri Rowstenkowski]

The image size is 2000*4096.