AIS quantification

Quantitative measures of the axon initial segment (AIS)
Updated 19 Sep 2014

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ais.m quantifies parameters of axon initial segment (AIS) location & length in appropriately immuno-stained neuronal tissue (see Grubb & Burrone 2010 Nature 465:1070). Can also produce indices of how well a particular probe or protein is localised to the AIS.
ais_z3.m (with input from Tom Watkins) maintains most features of ais.m, but now takes in a single RGB tif file as input, can analyse up to 3 separate fluorescence channels, gives measures of AIS fluorescence intensity, and gives more accurate measures of position along the axon.

ais_auto.m (written by Dennis Kruijssen) allows for semi-automatic measurement of AIS length when label is very clean, and can be combined into batch analysis with batch_ais_auto.m .

Cite As

Matthew Grubb (2024). AIS quantification (, MATLAB Central File Exchange. Retrieved .

MATLAB Release Compatibility
Created with R2014a
Compatible with any release
Platform Compatibility
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Version Published Release Notes

Added ais_auto.m and associated files for semi-automatic measurement of AIS length

Update April 2011: ais_z3.m and instructions for use included