Nuclei Counter

automatically count the number of nucleus in a microscope image

This script accept an input nuclei image taken under microscope, then preprocess the image with tophat and bottomhat filter, enhance its contrast by multiplying gamma. Convert it to binary image with a histogram based threshold, count the number of connected components and detect edges of nucleus with LoG filter, then plot edges. Or calculate distance transform and detect seed points,use watershed algorithm for segmentation.