Running the software for multiple images

I have this folder of images and would like to run my software on them. However, I am not so sure how and what to start with. Thanks in advance.

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Thank you so much! May I know how can I obtain an average image from a folder which contains multiple images?
for imagenumber = 1 : 20
TheImageArray = read image #imagenumber
if imagenumber == 1
image_total = double(TheImageArray)
else
image_total = image_total + TheImageArray
end
end
mean_Image = image_total / number of images

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