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Doubts about image processing

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Daniel
Daniel on 17 Mar 2011
Hello,
I am doing a research where i have to work with images from the corneal nerves, and they are really dark. These pictures where taken by a confocal microscopy. I am having difficulties to divide the pictures in blocks. I really need to do this because i want to apply the histogram to small regions and then do the segmentation. Can anyone help me?
Thank you for your time.
Daniel Carvalho

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Jan
Jan on 17 Mar 2011
Could you explain the difficulties you have with any details? What exactly means "really dark" - are they completely black? What kind of images are you working with? Can you post a small example of the images and the code?
Daniel
Daniel on 6 Apr 2011
the images that i am working is something like this http://www.iovs.org/content/48/5/2017/F1.small.gif.
I want to isolate the nerves (the white stripes). I am using filters like the median, the functions imadjust, adapthisteq, bwmorph, rangefilt...
I can't show you my code because it is really confusing.

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Accepted Answer

Sean de Wolski
Sean de Wolski on 6 Apr 2011
Perhaps Brett Shoelson's local Otsu utility would help you:

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More Answers (1)

Tim Zaman
Tim Zaman on 6 Apr 2011
I would recommend using photoshop (or equivalent) first to edit the image to a usable format, as photoshop in that matter is more user-friendly, though less scientific.

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